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KMID : 0613820070170030381
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Journal of Life Science
2007 Volume.17 No. 3 p.381 ~ p.385
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The expression of human Spt16 is associated with cell proliferation
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Gwak Jung-Sug
Cho Mun-Ju
Ryu Min-Jung
Oh Sang-Taek
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Abstract
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Facilitates chromatin transcription (FACT) is a chromatin-specific elongation factor required for transcription of chromatin templates in vivo and in vitro. FACT consists of human homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein (hSpt16) and the high mobility group-1-like protein structure- specific recognition protein-1 (SSRP-1). Here we show that the protein level of hSpt16 is massively down-regulated in quiescent T98G cells using both immunofluorescence and western blot analysis. In contrast, we observe high level of the hSpt16 expression in the proliferative T98G cells. Interestingly, the expression of SSRP-1 is not altered in both quiescent and proliferative states. Taken together, our findings implicate that the expression of hSpt16 is associated with the proliferative state and can be used as a proliferation marker.
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KEYWORD
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FACT, hSpt16, cell cycle, T98G cell
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